Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR2F2

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

GEO Accession
GSM1010782
antibody antibodydescription
Mouse monoclonal IgG, specific for an epitope mapping between amino acids 13-44 at the N-terminus of NR2F2 of human origin. Antibody Target: NR2F2
antibody targetdescription
This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. (provided by RefSeq)
antibody vendorid
sc-271940
antibody vendorname
Santa Cruz Biotechnology
antibody
NR2F2_(SC-271940)
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell organism
human
cell sex
F
cell
K562
controlid
SL6023
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL8577,SL8143
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010782: HudsonAlpha_ChipSeq_K562_NR2F2_(SC-271940)_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
85188065
Reads aligned (%)
85.4
Duplicates removed (%)
13.2
Number of peaks
33161 (qval < 1E-05)

hg19

Number of total reads
85188065
Reads aligned (%)
85.1
Duplicates removed (%)
13.6
Number of peaks
33168 (qval < 1E-05)

Base call quality data from DBCLS SRA