Antigen

Antigen Class

TFs and others

Antigen

SP2

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H1

NA

NA

Sample

GEO Accession

GSM1010776

antibody antibodydescription

Rabbit polyclonal IgG, epitope mapping near the N-terminus of SP2 of human origin. Antibody Target: SP2

antibody targetdescription

This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. (provided by RefSeq)

antibody vendorid

sc-643

antibody vendorname

Santa Cruz Biotechnology

antibody

SP2_(SC-643)

cell description

embryonic stem cells

cell karyotype

normal

cell lineage

inner cell mass

cell organism

human

cell sex

M

cell

H1-hESC

controlid

SL6018

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL6005,SL5690

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010776: HudsonAlpha_ChipSeq_H1-hESC_SP2_(SC-643)_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

83187817

Reads aligned (%)

79.1

Duplicates removed (%)

13.7

Number of peaks

3089 (qval < 1E-05)

hg19

Number of total reads

83187817

Reads aligned (%)

78.6

Duplicates removed (%)

14.6

Number of peaks

3114 (qval < 1E-05)