Antigen

Antigen Class

TFs and others

Antigen

TAF1

Cell type

Cell type Class

Neural

Cell type

SK-N-SH

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

GEO Accession

GSM1010773

antibody antibodydescription

Mouse monoclonal (p250). Antibody Target: TAF1

antibody targetdescription

TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa. Also designated TAF250.

antibody vendorid

sc-735

antibody vendorname

Santa Cruz Biotechnology

antibody

TAF1

cell description

neuroblastoma, newly promoted to tier 2: not in 2011 analysis, the SK-N-SH line was established from a neuroblastoma in a 4 year old individual.

cell karyotype

cancer

cell lineage

ectoderm

cell organism

human

cell sex

F

cell

SK-N-SH

controlid

SL2134

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL6462,SL5612

protocol description

2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)

protocol

v041610.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010773: HudsonAlpha_ChipSeq_SK-N-SH_TAF1_v041610.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

80444913

Reads aligned (%)

79.9

Duplicates removed (%)

24.5

Number of peaks

19056 (qval < 1E-05)

hg19

Number of total reads

80444913

Reads aligned (%)

79.3

Duplicates removed (%)

25.9

Number of peaks

18902 (qval < 1E-05)