Antigen

Antigen Class

TFs and others

Antigen

PML

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

GEO Accession

GSM1010771

antibody antibodydescription

Mouse monoclonal IgG, N-terminal epitope corresponding to residues 37-51 of PML of human origin. Antibody Target: PML

antibody targetdescription

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

antibody vendorid

sc-71910

antibody vendorname

Santa Cruz Biotechnology

antibody

PML_(SC-71910)

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

cell organism

human

cell sex

F

cell

GM12878

controlid

SL6022

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL7281,SL7831

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010771: HudsonAlpha_ChipSeq_GM12878_PML_(SC-71910)_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

82717228

Reads aligned (%)

79.3

Duplicates removed (%)

10.2

Number of peaks

28090 (qval < 1E-05)

hg19

Number of total reads

82717228

Reads aligned (%)

76.8

Duplicates removed (%)

8.8

Number of peaks

28886 (qval < 1E-05)