Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

GEO Accession
GSM1010761
antibody antibodydescription
Rabbit polyclonal. Antibody Target: RAD21
antibody targetdescription
Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14
antibody vendorid
ab992
antibody vendorname
abcam
antibody
Rad21
cell description
epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
cell karyotype
cancer
cell lineage
endoderm
cell organism
human
cell sex
M
cell
A549
controlid
SL13321,SL16753
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL12634,SL16300
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010761: HudsonAlpha_ChipSeq_A549_Rad21_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
54763105
Reads aligned (%)
84.5
Duplicates removed (%)
10.0
Number of peaks
48269 (qval < 1E-05)

hg38

Number of total reads
54763105
Reads aligned (%)
85.9
Duplicates removed (%)
9.0
Number of peaks
48487 (qval < 1E-05)

Base call quality data from DBCLS SRA