Antigen

Antigen Class

TFs and others

Antigen

CREB1

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

GEO Accession

GSM1010760

antibody antibodydescription

Mouse monoclonal IgG1; raised against amino acids 254-327 mapping within the DNA binding and dimerization domain of human CREB-1. Antibody Target: CREB1

antibody targetdescription

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)

antibody vendorid

sc-240

antibody vendorname

Santa Cruz Biotechnology

antibody

CREB1_(SC-240)

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

cell organism

human

cell sex

F

cell

GM12878

controlid

SL12789,SL12049

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL16741,SL12408

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010760: HudsonAlpha_ChipSeq_GM12878_CREB1_(SC-240)_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

54525404

Reads aligned (%)

85.8

Duplicates removed (%)

15.9

Number of peaks

42742 (qval < 1E-05)

hg19

Number of total reads

54525404

Reads aligned (%)

82.7

Duplicates removed (%)

14.1

Number of peaks

43946 (qval < 1E-05)