Antigen

Antigen Class

TFs and others

Antigen

TCF12

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

GEO Accession

GSM1010746

antibody antibodydescription

HEB (A-20) Rabbit polyclonal IgG, 200 ?g/ml. Epitope mapping within the C-terminus of HEB of human origin. Antibody Target: TCF12

antibody targetdescription

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

antibody vendorid

sc-357

antibody vendorname

Santa Cruz Biotechnology

antibody

TCF12

cell description

epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis

cell karyotype

cancer

cell lineage

endoderm

cell organism

human

cell sex

M

cell

A549

controlid

SL6021

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL8076,SL7114

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

1 h with 0.02% Ethanol (Myers)

treatment

EtOH_0.02pct

Sequenced DNA Library

library_name

GSM1010746: HudsonAlpha_ChipSeq_A549_TCF12_EtOH_0.02pct_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

69326773

Reads aligned (%)

85.9

Duplicates removed (%)

11.3

Number of peaks

33884 (qval < 1E-05)

hg19

Number of total reads

69326773

Reads aligned (%)

85.5

Duplicates removed (%)

12.2

Number of peaks

33852 (qval < 1E-05)