Antigen

Antigen Class

TFs and others

Antigen

TCF3

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

GEO Accession

GSM1010745

antibody antibodydescription

E2A.E12(V-18) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of E2A.E12 of human origin. Recommended for detection of E12 and E47 of m, r and h origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG. Antibody Target: TCF3

antibody targetdescription

Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.

antibody vendorid

sc-349

antibody vendorname

Santa Cruz Biotechnology

antibody

TCF3_(SC-349)

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

cell organism

human

cell sex

F

cell

GM12878

controlid

SL1394,SL1395

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL633,SL480

protocol description

one 15-cycle round of PCR (Myers)

protocol

PCR1x

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010745: HudsonAlpha_ChipSeq_GM12878_TCF3_(SC-349)_PCR1x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

52328399

Reads aligned (%)

58.7

Duplicates removed (%)

13.5

Number of peaks

21289 (qval < 1E-05)

hg19

Number of total reads

52328399

Reads aligned (%)

57.4

Duplicates removed (%)

13.6

Number of peaks

21740 (qval < 1E-05)