Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TCF3

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

GEO Accession
GSM1010745
antibody antibodydescription
E2A.E12(V-18) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of E2A.E12 of human origin. Recommended for detection of E12 and E47 of m, r and h origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG. Antibody Target: TCF3
antibody targetdescription
Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
antibody vendorid
sc-349
antibody vendorname
Santa Cruz Biotechnology
antibody
TCF3_(SC-349)
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
cell karyotype
normal
cell lineage
mesoderm
cell organism
human
cell sex
F
cell
GM12878
controlid
SL1394,SL1395
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL633,SL480
protocol description
one 15-cycle round of PCR (Myers)
protocol
PCR1x
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010745: HudsonAlpha_ChipSeq_GM12878_TCF3_(SC-349)_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
52328399
Reads aligned (%)
49.6
Duplicates removed (%)
13.6
Number of peaks
21740 (qval < 1E-05)

hg38

Number of total reads
52328399
Reads aligned (%)
50.8
Duplicates removed (%)
13.5
Number of peaks
21289 (qval < 1E-05)

Base call quality data from DBCLS SRA