Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SP4

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

GEO Accession
GSM1010743
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping at the C-terminus of Sp4 of human origin. Antibody Target: SP4
antibody targetdescription
Binds to GT and GC boxes promoters elements. Probable transcriptional activator.
antibody vendorid
sc-645
antibody vendorname
Santa Cruz Biotechnology
antibody
SP4_(V-20)
cell description
embryonic stem cells
cell karyotype
normal
cell lineage
inner cell mass
cell organism
human
cell sex
M
cell
H1-hESC
controlid
SL6018
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL7836,SL8135
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010743: HudsonAlpha_ChipSeq_H1-hESC_SP4_(V-20)_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
67023958
Reads aligned (%)
75.3
Duplicates removed (%)
9.3
Number of peaks
12498 (qval < 1E-05)

hg38

Number of total reads
67023958
Reads aligned (%)
76.1
Duplicates removed (%)
8.6
Number of peaks
13047 (qval < 1E-05)

Base call quality data from DBCLS SRA