Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

HL-60

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

GEO Accession

GSM1010737

antibody antibodydescription

Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target: POL2

antibody targetdescription

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes

antibody vendorid

ab5408

antibody vendorname

abcam

antibody

Pol2-4H8

cell description

promyelocytic leukemia cells, (PMID: 276884)

cell karyotype

cancer

cell lineage

mesoderm

cell organism

human

cell sex

F

cell

HL-60

controlid

SL12792

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL16322,SL12628

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010737: HudsonAlpha_ChipSeq_HL-60_Pol2-4H8_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

47613823

Reads aligned (%)

96.7

Duplicates removed (%)

6.6

Number of peaks

40657 (qval < 1E-05)

hg19

Number of total reads

47613823

Reads aligned (%)

96.1

Duplicates removed (%)

7.1

Number of peaks

40611 (qval < 1E-05)