Antigen

Antigen Class

TFs and others

Antigen

REST

Cell type

Cell type Class

Neural

Cell type

SK-N-SH

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

GEO Accession

GSM1010735

antibody antibodydescription

Mouse monoclonal. Antibody Target: NRSF

antibody targetdescription

Neuron-restrictive silencer transcription factor

antibody vendorid

(Made by Protein Expression Center: pec@caltech.edu)

antibody vendorname

David Anderson, Caltech

antibody

NRSF

cell description

neuroblastoma, newly promoted to tier 2: not in 2011 analysis, the SK-N-SH line was established from a neuroblastoma in a 4 year old individual.

cell karyotype

cancer

cell lineage

ectoderm

cell organism

human

cell sex

F

cell

SK-N-SH

controlid

SL3774

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL5896,SL5494

protocol description

2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)

protocol

v041610.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010735: HudsonAlpha_ChipSeq_SK-N-SH_NRSF_v041610.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg19

Number of total reads

62131282

Reads aligned (%)

81.4

Duplicates removed (%)

20.2

Number of peaks

22036 (qval < 1E-05)

hg38

Number of total reads

62131282

Reads aligned (%)

81.8

Duplicates removed (%)

19.4

Number of peaks

21613 (qval < 1E-05)