Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CREB1

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

GEO Accession
GSM1010726
antibody antibodydescription
Mouse monoclonal IgG1; raised against amino acids 254-327 mapping within the DNA binding and dimerization domain of human CREB-1. Antibody Target: CREB1
antibody targetdescription
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)
antibody vendorid
sc-240
antibody vendorname
Santa Cruz Biotechnology
antibody
CREB1_(SC-240)
cell description
epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
cell karyotype
cancer
cell lineage
endoderm
cell organism
human
cell sex
M
cell
A549
controlid
SL3898
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL3898
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
protocol
v041610.2
replicate
2
treatment description
1 h with 0.02% Ethanol (Myers)
treatment
EtOH_0.02pct

Sequenced DNA Library

library_name
GSM1010726: HudsonAlpha_ChipSeq_A549_CREB1_(SC-240)_EtOH_0.02pct_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
25435483
Reads aligned (%)
69.6
Duplicates removed (%)
17.0
Number of peaks
7680 (qval < 1E-05)

hg38

Number of total reads
25435483
Reads aligned (%)
71.5
Duplicates removed (%)
15.5
Number of peaks
7618 (qval < 1E-05)

Base call quality data from DBCLS SRA