Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
JUND

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

GEO Accession
GSM1010723
antibody antibodydescription
rabbit polyclonal IgG. Antibody Target: JunD
antibody targetdescription
The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. It has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternate translation initiation site usage results in the production of different isoforms. (provided by RefSeq)
antibody vendorid
sc-74
antibody vendorname
Santa Cruz Biotechnology
antibody
JunD
cell description
epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
cell karyotype
cancer
cell lineage
endoderm
cell organism
human
cell sex
M
cell
A549
controlid
SL4698,SL3900,SL3978
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL4698,SL3900
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
protocol
v041610.2
replicate
1,2
softwareversion
MACS
treatment description
1 h with 0.02% Ethanol (Myers)
treatment
EtOH_0.02pct

Sequenced DNA Library

library_name
GSM1010723: HudsonAlpha_ChipSeq_A549_JunD_EtOH_0.02pct_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
64957762
Reads aligned (%)
64.5
Duplicates removed (%)
22.2
Number of peaks
24938 (qval < 1E-05)

hg38

Number of total reads
64957762
Reads aligned (%)
65.9
Duplicates removed (%)
21.1
Number of peaks
25020 (qval < 1E-05)

Base call quality data from DBCLS SRA