Antigen

Antigen Class

TFs and others

Antigen

STAT5A

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

GEO Accession

GSM1010721

antibody antibodydescription

Mouse monoclonal IgG, recommended for the detection of Stat5a and Stat5b of human origin by WB, IP, IF and ELISA. Antibody Target: STAT5A

antibody targetdescription

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. (provided by RefSeq)

antibody vendorid

sc-74442

antibody vendorname

Santa Cruz Biotechnology

antibody

STAT5A_(SC-74442)

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

cell organism

human

cell sex

F

cell

GM12878

controlid

SL6022

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL7897,SL8127

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010721: HudsonAlpha_ChipSeq_GM12878_STAT5A_(SC-74442)_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

84148447

Reads aligned (%)

85.5

Duplicates removed (%)

7.4

Number of peaks

21507 (qval < 1E-05)

hg19

Number of total reads

84148447

Reads aligned (%)

83.3

Duplicates removed (%)

6.3

Number of peaks

22132 (qval < 1E-05)