Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H7
NA
NA

Attributes by original data submitter

Sample

source_name
H7-hESC
biomaterial_provider
WiCell
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
H7-hESC
cell organism
human
cell description
undifferentiated embryonic stem cells
cell lineage
inner cell mass
cell sex
U
antibody
H3K36me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K36me3
antibody targetdescription
Histone H3 (tri-methyl K36). Marks regions of RNAPII elongation, including coding and non-coding transcripts.
antibody vendorname
Abcam
antibody vendorid
ab9050
treatment
diffProtA_14d
treatment description
H7 embryoid bodies differentiation protocol for cardiomyocyte, endothelial, smooth muscle, with markers cardiac troponin T (cTnT), CD31/PECAM1, smooth muscle alpha actin (SMA), respectively, using proteins from bone morphogenic protein 4 (BMP4), activin A and basic fibroblast growth factor (bFGF) for 14 days, cell sorting and specific growth factors were used, lineage: mesoderm
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH002532
labexpid
DS14123
labversion
Bowtie 0.12.7
replicate
1

Sequenced DNA Library

library_name
GSM945324: UW_ChipSeq_H7-hESC_H3K36me3_diffProtA_14d
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
43890248
Reads aligned (%)
94.5
Duplicates removed (%)
5.0
Number of peaks
1436 (qval < 1E-05)

hg19

Number of total reads
43890248
Reads aligned (%)
94.0
Duplicates removed (%)
5.6
Number of peaks
1451 (qval < 1E-05)

Base call quality data from DBCLS SRA