Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H7

NA

NA

Sample

source_name

H7-hESC

biomaterial_provider

WiCell

donor_id

ENCDO221AAA

biomaterial_type

in vitro differentiated cells

cell_type

cardiac mesoderm

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

H7-hESC

cell organism

human

cell description

undifferentiated embryonic stem cells

cell lineage

inner cell mass

cell sex

U

antibody

H3K4me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K4me3

antibody targetdescription

Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.

antibody vendorname

Millipore

antibody vendorid

07-473

treatment

diffProtA_5d

treatment description

H7 embryoid bodies differentiation protocol for cardiomyocyte, endothelial, smooth muscle, with markers cardiac troponin T (cTnT), CD31/PECAM1, smooth muscle alpha actin (SMA), respectively, using proteins from bone morphogenic protein 4 (BMP4), activin A and basic fibroblast growth factor (bFGF) for 5 days, lineage: mesoderm

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH002544

labexpid

DS13777

labversion

Bowtie 0.12.7

replicate

1

Sequenced DNA Library

library_name

GSM945322: UW_ChipSeq_H7-hESC_H3K4me3_diffProtA_5d

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

39876422

Reads aligned (%)

88.6

Duplicates removed (%)

33.1

Number of peaks

36945 (qval < 1E-05)

hg19

Number of total reads

39876422

Reads aligned (%)

88.3

Duplicates removed (%)

33.5

Number of peaks

37401 (qval < 1E-05)