Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H7

NA

NA

Sample

source_name

H7-hESC

biomaterial_provider

WiCell

donor_id

ENCDO221AAA

biomaterial_type

in vitro differentiated cells

cell_type

cardiac mesoderm

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

H7-hESC

cell organism

human

cell description

undifferentiated embryonic stem cells

cell lineage

inner cell mass

cell sex

U

antibody

H3K4me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K4me3

antibody targetdescription

Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.

antibody vendorname

Millipore

antibody vendorid

07-473

treatment

diffProtA_14d

treatment description

H7 embryoid bodies differentiation protocol for cardiomyocyte, endothelial, smooth muscle, with markers cardiac troponin T (cTnT), CD31/PECAM1, smooth muscle alpha actin (SMA), respectively, using proteins from bone morphogenic protein 4 (BMP4), activin A and basic fibroblast growth factor (bFGF) for 14 days, cell sorting and specific growth factors were used, lineage: mesoderm

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH002532

labexpid

DS14121

labversion

Bowtie 0.12.7

replicate

1

Sequenced DNA Library

library_name

GSM945321: UW_ChipSeq_H7-hESC_H3K4me3_diffProtA_14d

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

48352697

Reads aligned (%)

90.0

Duplicates removed (%)

32.8

Number of peaks

36553 (qval < 1E-05)

hg19

Number of total reads

48352697

Reads aligned (%)

89.7

Duplicates removed (%)

33.3

Number of peaks

36464 (qval < 1E-05)