Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
Monocytes-CD14+
Tissue
monocytes
Lineage
mesoderm
Description
Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Attributes by original data submitter

Sample

source_name
Monocytes-CD14+_RO01746
biomaterial_provider
S. Heimfeld Laboratory, FHCRC
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
Monocytes-CD14+_RO01746
cell organism
human
cell description
Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), Monocytes-CD14+_RO01746 and Monocytes-CD14+_RO01826 are being used as replicates, newly promoted to tier 2: not in 2011 analysis
cell karyotype
normal
cell lineage
mesoderm
cell sex
F
antibody
H3K27me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27me3
antibody targetdescription
Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.
antibody vendorname
Millipore
antibody vendorid
07-449
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH002169
labexpid
DS19094
labversion
Bowtie 0.12.7
replicate
1

Sequenced DNA Library

library_name
GSM945301: UW_ChipSeq_Monocytes-CD14+_RO01746_H3K27me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
28346671
Reads aligned (%)
97.0
Duplicates removed (%)
1.7
Number of peaks
2686 (qval < 1E-05)

hg19

Number of total reads
28346671
Reads aligned (%)
96.5
Duplicates removed (%)
2.4
Number of peaks
2681 (qval < 1E-05)

Base call quality data from DBCLS SRA