Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

biomaterial_provider

ATCC

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

K562

cell organism

human

cell description

leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC

cell karyotype

cancer

cell lineage

mesoderm

cell sex

F

antibody

H3K4me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K4me3

antibody targetdescription

Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.

antibody vendorname

Millipore

antibody vendorid

07-473

treatment

ZNF_knockout_A4.1-C6

treatment description

off-target control (Stam)

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000471

labexpid

DS15834

labversion

Bowtie 0.12.7

replicate

1

Sequenced DNA Library

library_name

GSM945298: UW_ChipSeq_K562_H3K4me3_ZNF_knockout_A4.1-C6

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

45256238

Reads aligned (%)

97.1

Duplicates removed (%)

23.9

Number of peaks

46140 (qval < 1E-05)

hg19

Number of total reads

45256238

Reads aligned (%)

96.8

Duplicates removed (%)

24.5

Number of peaks

47963 (qval < 1E-05)