Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
antibody
H3K4me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me3
antibody targetdescription
Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.
antibody vendorname
Millipore
antibody vendorid
07-473
treatment
ZNF_knockout_F4.1-B2
treatment description
beta-globin LCR deletion (18Kb), heterozygous (Stam)
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000471
labexpid
DS16129
labversion
Bowtie 0.12.7
replicate
1

Sequenced DNA Library

library_name
GSM945278: UW_ChipSeq_K562_H3K4me3_ZNF_knockout_F4.1-B2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
50658689
Reads aligned (%)
96.0
Duplicates removed (%)
29.4
Number of peaks
37747 (qval < 1E-05)

hg19

Number of total reads
50658689
Reads aligned (%)
95.7
Duplicates removed (%)
29.9
Number of peaks
37851 (qval < 1E-05)

Base call quality data from DBCLS SRA