Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Jurkat

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

Jurkat

biomaterial_provider

ATCC

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

Jurkat

cell organism

human

cell description

T lymphoblastoid derived from an acute T cell leukemia, "The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM." - ATCC. (PMID: 68013)

cell karyotype

cancer

cell lineage

mesoderm

cell sex

M

antibody

H3K4me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K4me3

antibody targetdescription

Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.

antibody vendorname

Millipore

antibody vendorid

07-473

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000966

labexpid

DS16197

labversion

Bowtie 0.12.7

replicate

1

Sequenced DNA Library

library_name

GSM945267: UW_ChipSeq_Jurkat_H3K4me3

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

60925620

Reads aligned (%)

98.7

Duplicates removed (%)

30.3

Number of peaks

34084 (qval < 1E-05)

hg19

Number of total reads

60925620

Reads aligned (%)

98.3

Duplicates removed (%)

30.9

Number of peaks

34021 (qval < 1E-05)