Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Lung
Cell type
NHLF
Tissue
lung
Lineage
endoderm
Description
lung fibroblasts

Attributes by original data submitter

Sample

source_name
NHLF
biomaterial_provider
Lonza
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
NHLF
cell organism
human
cell description
lung fibroblasts
cell karyotype
normal
cell lineage
endoderm
cell sex
U
antibody
H3K4me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me3
antibody targetdescription
Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.
antibody vendorname
Millipore
antibody vendorid
07-473
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH001899
labexpid
DS16351
labversion
Bowtie 0.12.7
replicate
1

Sequenced DNA Library

library_name
GSM945262: UW_ChipSeq_NHLF_H3K4me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
46418079
Reads aligned (%)
98.5
Duplicates removed (%)
20.4
Number of peaks
34411 (qval < 1E-05)

hg19

Number of total reads
46418079
Reads aligned (%)
98.2
Duplicates removed (%)
20.9
Number of peaks
34475 (qval < 1E-05)

Base call quality data from DBCLS SRA