Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

source_name

A549

biomaterial_provider

ATCC

biomaterial_type

immortalized cell line

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

line

A549

cell organism

human

cell description

epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis

cell karyotype

cancer

cell lineage

endoderm

cell sex

M

antibody

H3K4me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K4me3

antibody targetdescription

Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.

antibody vendorname

Millipore

antibody vendorid

07-473

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH001904

labexpid

DS18305

labversion

Bowtie 0.12.7

replicate

1

Sequenced DNA Library

library_name

GSM945244: UW_ChipSeq_A549_H3K4me3

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

35478061

Reads aligned (%)

96.6

Duplicates removed (%)

21.2

Number of peaks

36260 (qval < 1E-05)

hg19

Number of total reads

35478061

Reads aligned (%)

96.2

Duplicates removed (%)

21.5

Number of peaks

36181 (qval < 1E-05)