Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
antibody
H3K27me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27me3
antibody targetdescription
Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.
antibody vendorname
Millipore
antibody vendorid
07-449
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000471
labexpid
DS12066
labversion
Bowtie 0.12.5
replicate
1

Sequenced DNA Library

library_name
GSM945228: UW_ChipSeq_K562_H3K27me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
32926175
Reads aligned (%)
69.0
Duplicates removed (%)
33.7
Number of peaks
227 (qval < 1E-05)

hg19

Number of total reads
32926175
Reads aligned (%)
68.6
Duplicates removed (%)
35.0
Number of peaks
373 (qval < 1E-05)

Base call quality data from DBCLS SRA