Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
HL-60
biomaterial_provider
ATCC
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HL-60
cell organism
human
cell description
promyelocytic leukemia cells, (PMID: 276884)
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
antibody
H3K4me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me3
antibody targetdescription
Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.
antibody vendorname
Millipore
antibody vendorid
07-473
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000465
labexpid
DS11795
labversion
Bowtie 0.12.5
replicate
1

Sequenced DNA Library

library_name
GSM945222: UW_ChipSeq_HL-60_H3K4me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
29691032
Reads aligned (%)
76.7
Duplicates removed (%)
22.8
Number of peaks
27345 (qval < 1E-05)

hg19

Number of total reads
29691032
Reads aligned (%)
76.6
Duplicates removed (%)
23.0
Number of peaks
27317 (qval < 1E-05)

Base call quality data from DBCLS SRA