Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
GM12878
cell organism
human
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
cell karyotype
normal
cell lineage
mesoderm
cell sex
F
antibody
H3K36me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K36me3
antibody targetdescription
Histone H3 (tri-methyl K36). Marks regions of RNAPII elongation, including coding and non-coding transcripts.
antibody vendorname
Abcam
antibody vendorid
ab9050
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000463
labexpid
DS12186
labversion
Bowtie 0.12.5
replicate
1

Sequenced DNA Library

library_name
GSM945212: UW_ChipSeq_GM12878_H3K36me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
35535400
Reads aligned (%)
97.3
Duplicates removed (%)
3.0
Number of peaks
772 (qval < 1E-05)

hg19

Number of total reads
35535400
Reads aligned (%)
96.8
Duplicates removed (%)
3.7
Number of peaks
900 (qval < 1E-05)

Base call quality data from DBCLS SRA