Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H7
NA
NA

Attributes by original data submitter

Sample

source_name
H7-hESC
biomaterial_provider
WiCell
donor_id
ENCDO221AAA
biomaterial_type
in vitro differentiated cells
cell_type
cardiac mesoderm
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
H7-hESC
cell organism
human
cell description
undifferentiated embryonic stem cells
cell lineage
inner cell mass
cell sex
U
antibody
H3K4me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me3
antibody targetdescription
Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.
antibody vendorname
Millipore
antibody vendorid
07-473
treatment
diffProtA_2d
treatment description
H7 embryoid bodies differentiation protocol for cardiomyocyte, endothelial, smooth muscle, with markers cardiac troponin T (cTnT), CD31/PECAM1, smooth muscle alpha actin (SMA), respectively, using proteins from bone morphogenic protein 4 (BMP4), activin A and basic fibroblast growth factor (bFGF) for 2 days, lineage: mesoderm
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH002543
labexpid
DS14704
labversion
Bowtie 0.12.7
replicate
1

Sequenced DNA Library

library_name
GSM945191: UW_ChipSeq_H7-hESC_H3K4me3_diffProtA_2d
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
49552800
Reads aligned (%)
94.5
Duplicates removed (%)
23.9
Number of peaks
39876 (qval < 1E-05)

hg19

Number of total reads
49552800
Reads aligned (%)
94.1
Duplicates removed (%)
24.5
Number of peaks
41784 (qval < 1E-05)

Base call quality data from DBCLS SRA