Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H7
NA
NA

Attributes by original data submitter

Sample

source_name
H7-hESC
biomaterial_provider
WiCell
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
H7-hESC
cell organism
human
cell description
undifferentiated embryonic stem cells
cell lineage
inner cell mass
cell sex
U
antibody
H3K36me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K36me3
antibody targetdescription
Histone H3 (tri-methyl K36). Marks regions of RNAPII elongation, including coding and non-coding transcripts.
antibody vendorname
Abcam
antibody vendorid
ab9050
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000926
labexpid
DS14115
labversion
Bowtie 0.12.5
replicate
1

Sequenced DNA Library

library_name
GSM945184: UW_ChipSeq_H7-hESC_H3K36me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
45019770
Reads aligned (%)
91.2
Duplicates removed (%)
4.6
Number of peaks
1351 (qval < 1E-05)

hg19

Number of total reads
45019770
Reads aligned (%)
90.6
Duplicates removed (%)
5.1
Number of peaks
1336 (qval < 1E-05)

Base call quality data from DBCLS SRA