Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HUVEC
biomaterial_provider
Lonza
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HUVEC
cell organism
human
cell description
umbilical vein endothelial cells
cell karyotype
normal
cell lineage
mesoderm
cell sex
U
antibody
H3K27me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27me3
antibody targetdescription
Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.
antibody vendorname
Millipore
antibody vendorid
07-449
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000468
labexpid
DS12227
labversion
Bowtie 0.12.5
replicate
1

Sequenced DNA Library

library_name
GSM945180: UW_ChipSeq_HUVEC_H3K27me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
34527427
Reads aligned (%)
95.9
Duplicates removed (%)
4.9
Number of peaks
1800 (qval < 1E-05)

hg19

Number of total reads
34527427
Reads aligned (%)
95.5
Duplicates removed (%)
5.6
Number of peaks
1836 (qval < 1E-05)

Base call quality data from DBCLS SRA