Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Kidney
Cell type
HRE
Tissue
epithelium
Lineage
mesoderm
Description
renal epithelial cells

Attributes by original data submitter

Sample

source_name
HRE
biomaterial_provider
Lonza
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HRE
cell organism
human
cell description
renal epithelial cells
cell karyotype
normal
cell lineage
mesoderm
cell sex
U
antibody
H3K27me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27me3
antibody targetdescription
Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.
antibody vendorname
Millipore
antibody vendorid
07-449
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000467
labexpid
DS12422
labversion
Bowtie 0.12.5
replicate
1

Sequenced DNA Library

library_name
GSM945160: UW_ChipSeq_HRE_H3K27me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
74462891
Reads aligned (%)
49.3
Duplicates removed (%)
6.3
Number of peaks
6025 (qval < 1E-05)

hg19

Number of total reads
74462891
Reads aligned (%)
49.1
Duplicates removed (%)
6.9
Number of peaks
6064 (qval < 1E-05)

Base call quality data from DBCLS SRA