Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TFAP4

Cell type

Cell type Class
Digestive tract
Cell type
COLO 320
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Colorectal cancer cell line
cell line
COLO320
cell type
Colorectal cancer cell line
genetic modification
none
library strategy
transcription factor ChIP-seq
chip antibody
anti-TFAP4 (Santa Cruz Biotechnology, sc-18593X)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were purified using QIA quick column (DNase I-seq, ATAC-seq, Targeted RNA-/DNA-seq, and 4C-seq), QIA minElute column (ChIP-seq) or SPRI beads (ATAC-seq). Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
54119851
Reads aligned (%)
81.0
Duplicates removed (%)
15.9
Number of peaks
58874 (qval < 1E-05)

hg38

Number of total reads
54119851
Reads aligned (%)
82.1
Duplicates removed (%)
15.2
Number of peaks
58901 (qval < 1E-05)

Base call quality data from DBCLS SRA