Antigen

Antigen Class

TFs and others

Antigen

TFAP4

Cell type

Cell type Class

Digestive tract

Cell type

SNU-1750

NA

NA

Sample

source_name

Colorectal cancer cell line

cell line

SNU175

cell type

Colorectal cancer cell line

genetic modification

none

library strategy

transcription factor ChIP-seq

chip antibody

anti-TFAP4 (Santa Cruz Biotechnology, sc-18593X)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Libraries were purified using QIA quick column (DNase I-seq, ATAC-seq, Targeted RNA-/DNA-seq, and 4C-seq), QIA minElute column (ChIP-seq) or SPRI beads (ATAC-seq). Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

50560541

Reads aligned (%)

90.0

Duplicates removed (%)

47.2

Number of peaks

42764 (qval < 1E-05)

hg19

Number of total reads

50560541

Reads aligned (%)

89.5

Duplicates removed (%)

48.0

Number of peaks

42777 (qval < 1E-05)