Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Colorectal cancer cell line
cell line
HCT116
cell type
Colorectal cancer cell line
genetic modification
none
library strategy
histone ChIP-seq
chip antibody
anti-H3K27ac (Diagenode, C15410196)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were purified using QIA quick column (DNase I-seq, ATAC-seq, Targeted RNA-/DNA-seq, and 4C-seq), QIA minElute column (ChIP-seq) or SPRI beads (ATAC-seq). Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
95346135
Reads aligned (%)
99.0
Duplicates removed (%)
77.0
Number of peaks
16722 (qval < 1E-05)

hg19

Number of total reads
95346135
Reads aligned (%)
98.3
Duplicates removed (%)
78.4
Number of peaks
16657 (qval < 1E-05)

Base call quality data from DBCLS SRA