Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Kat2a

Cell type

Cell type Class
Blood
Cell type
MEL
Tissue
Blood
Lineage
cellLine
Description
Leukemia (K562 analog)

Attributes by original data submitter

Sample

source_name
MEL
biomaterial_provider
Weissman lab
lab
Stanford-m
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
MEL
cell organism
mouse
cell description
Leukemia (K562 analog)
cell sex
M
antibody
GCN5
antibody antibodydescription
Rabbit polyclonal anti-GCN5 antibody (2676), Immunogen : synthetic peptide coupled to Ovalbumin (sequence-MAEPSQAPTPAPAAQPRPLC). Antibody Target: GCN5
antibody targetdescription
KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. Acetylation of histones gives a specific tag for epigenetic transcription activation. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Component of the SAGA and ATAC complexes, complexes with histone acetyltransferase activities on histones H3 and H4
antibody vendorname
Laszlo Tora
antibody vendorid
Missing
treatment
None
treatment description
No special treatment or protocol applies
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
age
immortalized
age description
Immortal cells
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEM001947
strain
Unknown
strain description
Unknown strain origin

Sequenced DNA Library

library_name
GSM1003804: Stanford_ChipSeq_MEL_GCN5_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
60389800
Reads aligned (%)
96.0
Duplicates removed (%)
14.1
Number of peaks
16058 (qval < 1E-05)

mm9

Number of total reads
60389800
Reads aligned (%)
95.8
Duplicates removed (%)
14.0
Number of peaks
16104 (qval < 1E-05)

Base call quality data from DBCLS SRA