Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Zkscan1

Cell type

Cell type Class
Blood
Cell type
CH12
Tissue
Blood
Lineage
cellLine
Description
B-cell lymphoma (GM12878 analog)

Attributes by original data submitter

Sample

source_name
CH12
biomaterial_provider
Weissman lab
lab
Stanford-m
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
CH12
cell organism
mouse
cell description
B-cell lymphoma (GM12878 analog)
cell sex
F
antibody
ZKSCAN1_(HPA006672)
antibody antibodydescription
Rabbit polyclonal, Immunogen: Zinc finger with KRAB and SCAN domain-containing protein 1 recombinant protein epitope signature tag (PrEST). Antibody Target: ZKSCAN1
antibody targetdescription
The ZKSCAN1 gene encodes a transcriptional regulator of the KRAB (Kruppel-associated box) subfamily of zinc finger proteins, which contain repeated Cys2-His2 (C2H2) zinc finger domains that are connected by conserved sequences, called H/C links. Transcriptional regulatory proteins containing tandemly repeated zinc finger domains are thought to be involved in both normal and abnormal cellular proliferation and differentiation.
antibody vendorname
Sigma-Aldrich
antibody vendorid
HPA006672
treatment
None
treatment description
No special treatment or protocol applies
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
age
immortalized
age description
Immortal cells
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEM001939
labversion
PeakSeq1.0
strain
B10.H-2aH-4bp/Wts
strain description
Derived by inbreeding from selected F2 progeny of B10.A X B10.129

Sequenced DNA Library

library_name
GSM1003782: Stanford_ChipSeq_CH12_ZKSCAN1_(HPA006672)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
50679381
Reads aligned (%)
95.8
Duplicates removed (%)
15.0
Number of peaks
10030 (qval < 1E-05)

mm9

Number of total reads
50679381
Reads aligned (%)
95.5
Duplicates removed (%)
15.0
Number of peaks
10347 (qval < 1E-05)

Base call quality data from DBCLS SRA