Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SIRT3

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
U2OS_UV_SIRT3
cell line
U2OS
cell type
human osteosarcoma cells
treatment
UV-irradiated
chip antibody
SIRT3
chip antibody vendor
Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
27983248
Reads aligned (%)
67.5
Duplicates removed (%)
68.3
Number of peaks
221 (qval < 1E-05)

hg38

Number of total reads
27983248
Reads aligned (%)
68.2
Duplicates removed (%)
66.5
Number of peaks
206 (qval < 1E-05)

Base call quality data from DBCLS SRA