Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

A172LoxP ESC line

cell type

Chx10-ESC-derived motor neurons

chip-antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysates were sonicated and the DNA complexes were precipitated with rabbit anti-Chx10. Libraries were prepared according to the Illumina TruSeq protocol, validated using the bioanalyzer and real-time PCR, and then sequenced on the Illumina HiSeq 2000

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

34826145

Reads aligned (%)

61.3

Duplicates removed (%)

6.4

Number of peaks

276 (qval < 1E-05)

mm9

Number of total reads

34826145

Reads aligned (%)

61.3

Duplicates removed (%)

6.4

Number of peaks

217 (qval < 1E-05)