GSM2218595: Input DNA Rep2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
B cells
NA
NA
Attributes by original data submitter
Sample
source_name
Spleen
strain
C57BL/6
cell type
Primary splenic B cells
chip antibody
None
genotype
wild-type
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Splenic B cells isolated using the Stem Cell Technologies EasySep Mouse B cell isolation kit were rested for 1-2 hours in complete media (RPMI-1640 plus 10% fetal bovine serum, 1X Glutamax, 1X penicillin-streptomycin and 50 micromolar beta-mercaptoethanol) in a tissue culture incubator at 37C. After resting, cells were crosslinked with 0.25% formaldehyde for 8 minutes. Fixed B cells were lysed, chromatin were prepared and immunoprecipitated according to the protocol of ChIP-IT High Sensitivity Kit (Active Motif). ChIP-seq libraries were prepared using the manufracturer's protocol of Rubicon ThruPlex kit