GSM2218835: SMAD2/3, MCF10A MII, TGFβ 1.5h ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease
Attributes by original data submitter
Sample
source_name
Breast epithelial cell line, MCF10A MII
cell line
MCF10A MII
cell type
Breast epithelial cell line
transformation
H-Ras transformed
treatment
TGFβ3, 5 ng/ml, 1.5h
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin isolation, sonication and immunoprecipitation using anti-SMAD2/3 antibody (#610843, BD Transduction Laboratories) were performed essentially as described (Isogaya et al., 2014; Morikawa et al., 2016). The library was prepared using NEBNext ChIP-Seq Library Prep Reagent Set for Illumina (New England Biolabs) or KAPA DNA Library Preparation Kits for Illumina (KAPA Biosystems).