Curated Sample Data


Genome
hg19
Antigen Class
Unclassified
Antigen
Unclassified
Cell type Class
Blood
Cell type
Jurkat

Cell type information


Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by Original Data Submitter


source_name
T-ALL
cell type
Jurkat T-ALL cells
treated with
DMSO 4 hrs
young_id
20160406_4620

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified. Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
27448757
Reads aligned (%)
75.3
Duplicates removed (%)
17.7
Number of peaks
4119 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA