Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Others

Cell type

Fibroblasts

NA

NA

Sample

source_name

Fibroblast cell line, empty vector added

cell line

7250

cell type

fibroblast

antibody

NA

enrichment target

DNase-sensitivity

target function

Open Chromatin

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

14795912

Reads aligned (%)

93.4

Duplicates removed (%)

33.6

Number of peaks

2457 (qval < 1E-05)

hg19

Number of total reads

14795912

Reads aligned (%)

92.4

Duplicates removed (%)

34.0

Number of peaks

2139 (qval < 1E-05)