Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Muscle
Cell type
RMS209
NA
NA

Attributes by original data submitter

Sample

source_name
RMS primary tumor, PAX3-FOXO1, MYCN amp
cell line
RMS209
cell type
rhabdomyosarcoma
antibody
.
enrichment target
input DNA
target function
control

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
12764668
Reads aligned (%)
98.5
Duplicates removed (%)
23.8
Number of peaks
487 (qval < 1E-05)

hg19

Number of total reads
12764668
Reads aligned (%)
97.8
Duplicates removed (%)
25.2
Number of peaks
481 (qval < 1E-05)

Base call quality data from DBCLS SRA