Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Muscle
Cell type
Rh-4
NA
NA

Attributes by original data submitter

Sample

source_name
RMS cell line, PAX3-FOXO1
cell line
RH4
cell type
rhabdomyosarcoma
antibody
p300 (Santa Cruz, cat. #sc-585)
enrichment target
p300
target function
Acetylated Lysine Writer, Active enhancers

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
15684455
Reads aligned (%)
87.9
Duplicates removed (%)
9.0
Number of peaks
17942 (qval < 1E-05)

hg38

Number of total reads
15684455
Reads aligned (%)
89.0
Duplicates removed (%)
8.3
Number of peaks
18009 (qval < 1E-05)

Base call quality data from DBCLS SRA