Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Muscle
Cell type
Rh-4
NA
NA

Attributes by original data submitter

Sample

source_name
RMS cell line, PAX3-FOXO1
cell line
RH4
cell type
rhabdomyosarcoma
antibody
H3K4me3, Active Motif (cat39159 pAb)
enrichment target
H3K4me3
target function
Active genes/promoters

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
37813504
Reads aligned (%)
94.8
Duplicates removed (%)
19.9
Number of peaks
30063 (qval < 1E-05)

hg19

Number of total reads
37813504
Reads aligned (%)
94.4
Duplicates removed (%)
20.4
Number of peaks
29864 (qval < 1E-05)

Base call quality data from DBCLS SRA