Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Muscle
Cell type
Rh-4
NA
NA

Attributes by original data submitter

Sample

source_name
RMS cell line, PAX3-FOXO1
cell line
RH4
cell type
rhabdomyosarcoma
antibody
BRD4, Bethyl A301-985A50
enrichment target
BRD4
target function
Acetylated Lysine Reader, Active Enhancers

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
35217260
Reads aligned (%)
86.8
Duplicates removed (%)
10.4
Number of peaks
2454 (qval < 1E-05)

hg38

Number of total reads
35217260
Reads aligned (%)
89.9
Duplicates removed (%)
8.0
Number of peaks
2731 (qval < 1E-05)

Base call quality data from DBCLS SRA