Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Muscle
Cell type
RD
Primary Tissue
Skelatal Muscle
Tissue Diagnosis
Rhabdomyosarcoma

Attributes by original data submitter

Sample

source_name
RMS cell line, NRAS
cell line
RD
cell type
rhabdomyosarcoma
antibody
.
enrichment target
input DNA
target function
control

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
15628054
Reads aligned (%)
93.7
Duplicates removed (%)
9.0
Number of peaks
1895 (qval < 1E-05)

hg19

Number of total reads
15628054
Reads aligned (%)
91.2
Duplicates removed (%)
14.9
Number of peaks
1400 (qval < 1E-05)

Base call quality data from DBCLS SRA