Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

ML-2

Primary Tissue

Bone Marrow

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Leukemia cell line (ML-2)

cell line

Leukemia cell line; ML-2

cell type

Adult monocytic cell line derived from AML M4 with t(6;11)(q27;q23) translocation

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq: Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated and protein-DNA complexes were isolated with antibody. Nascent RNA-seq: 108 4sU-treated cells were lysed in 10ml Trizol. RNA was extracted using chloroform and precipitated with EtOH. 4sU RNA was biotinylated with 1mg/ml Biotin-HPDP and nascent RNA was enriched for using streptavidin-coated beads. Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040) or Ultra RNA Library Prep Master Mix Set (Part# E7530).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

42306363

Reads aligned (%)

98.3

Duplicates removed (%)

2.5

Number of peaks

1126 (qval < 1E-05)

hg19

Number of total reads

42306363

Reads aligned (%)

97.3

Duplicates removed (%)

2.8

Number of peaks

778 (qval < 1E-05)