Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CBX3

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Mouse monoclonal IgG, raised against recombinant CBX3 of human origin. Antibody Target: CBX3
antibody targetdescription
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-101004
controlid
wgEncodeEH000052
replicate
1
softwareversion
ScriptureVPaperR3
cell sex
F
antibody
CBX3_(SC-101004)
antibody antibodydescription
Mouse monoclonal IgG, raised against recombinant CBX3 of human origin. Antibody Target: CBX3
antibody targetdescription
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-101004
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
K562/None/Input/std
labversion
Illumina_GA2x
labversion description
Illumina Genome Analyzer IIx
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003568: Broad_ChipSeq_K562_CBX3_(SC-101004)
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
48038216
Reads aligned (%)
74.6
Duplicates removed (%)
12.8
Number of peaks
1877 (qval < 1E-05)

hg38

Number of total reads
48038216
Reads aligned (%)
78.0
Duplicates removed (%)
10.2
Number of peaks
2058 (qval < 1E-05)

Base call quality data from DBCLS SRA