Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Blood

Cell type

Monocytes-CD14+

Tissue

monocytes

Lineage

mesoderm

Description

Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Sample

source_name

Monocytes-CD14+_RO01746

biomaterial_provider

S. Heimfeld Laboratory, FHCRC

donor_id

ENCDO265AAA

biomaterial_type

primary cell

cell_type

CD14-positive monocyte

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K36me3

antibody targetdescription

Specific for histone H3 tri methylated at lysine 36, weakly reacts with H3K36me2. Marks regions of RNAPII elongation, including coding and non-coding transcripts.

antibody vendorname

abcam

antibody vendorid

ab9050

controlid

wgEncodeEH003130

replicate

1,2

softwareversion

ScriptureVPaperR3

cell sex

F

antibody

H3K36me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K36me3

antibody targetdescription

Histone H3 (tri-methyl K36). Marks regions of RNAPII elongation, including coding and non-coding transcripts.

antibody vendorname

Abcam

antibody vendorid

ab9050

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

Monocytes-CD14+_RO01746/Input/std

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM1003562: Broad_ChipSeq_Monocytes-CD14+_RO01746_H3K36me3

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

47700731

Reads aligned (%)

98.7

Duplicates removed (%)

10.8

Number of peaks

6431 (qval < 1E-05)

hg19

Number of total reads

47700731

Reads aligned (%)

98.2

Duplicates removed (%)

11.7

Number of peaks

6399 (qval < 1E-05)