Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Monocytes-CD14+
Tissue
monocytes
Lineage
mesoderm
Description
Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Attributes by original data submitter

Sample

source_name
Monocytes-CD14+_RO01746
biomaterial_provider
S. Heimfeld Laboratory, FHCRC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription
Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname
abcam
antibody vendorid
ab4729
controlid
wgEncodeEH003130
replicate
1,2
softwareversion
ScriptureVPaperR3
cell sex
F
antibody
H3K27ac
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription
Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname
Abcam
antibody vendorid
ab4729
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
Monocytes-CD14+_RO01746/Input/std
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003559: Broad_ChipSeq_Monocytes-CD14+_RO01746_H3K27ac
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
43389504
Reads aligned (%)
98.9
Duplicates removed (%)
8.5
Number of peaks
42081 (qval < 1E-05)

hg19

Number of total reads
43389504
Reads aligned (%)
98.7
Duplicates removed (%)
8.7
Number of peaks
42101 (qval < 1E-05)

Base call quality data from DBCLS SRA